Yeast Lab-Cell Respiration
Abstract- In this lab my partner and I investigate the effects of temperature on the rate of cell respiration of yeast. Fortubes are tested in hot water, warm beads, room temperature and an Ice bath. The results were that the hot water bath rocketed up and produced a rate so fast that the stopper actually popped off the tube after only 2 minutes. The warm bath actually came in under the room temperature due to a possible error in the setup in the lab. The ice bath either slowed down so much it was not able to be recorded or stopped respiration completely.
Intro
Question- How does temperature affect cell respiration?
Background- Cell respiration is a complicated topic, as it is very complex and has many different parts. The basic equation of the process in animal cells is C6H12O6 + 6O2 yields 6CO2 + 6H2O + 36-38 ATP and in plant cells is the opposite, where they yield sugar and oxygen. In Cell Respiration for humans, food is taken in and broken down, and energy is released to create ATP to be used for other processes.
Hypothesis- If four test tubes with yeast are tested for the rate of their cell respiration based on the amount of gas which is produced with one at room temperature, one in ice, one at 50C and one at 70C, then the warmer conditions, the faster the cell respiration will be.
Methodology
Materials- 4 test tubes, four tube plugs, four gas recoding syringes, ice box, bead warmer, hot plate with beaker and water, yeast, warm water, salt and sugar
Procedure-
- Prepare the 4 test tubes with 1g of sugar, 35mL of warm water, .1g of salt and 1g of yeast.
- Put the flat caps on and shake to mix.
- Put the new plug with syringe on and let them sit for 5 minutes allowing the process to start.
- Take an initial reading after putting the tubes in their four locations (hot water bath, warm bead bath, ice bath, and room temp) by pushing the syringe to 0 and letting it come back out.
- Repeat the reading process in 1 minute intervals at least 5 times.
Results
Room Temp (control)
|
Ice Bath (10C)
|
Bead Bath (50C)
|
Hot Bath (70C)
|
2.8
|
1.1
|
1.0
|
2.0
|
3.8
|
2.1
|
2.0
|
13.0
|
6.0
|
2.4
|
2.8
|
max
|
8.0
|
2.4
|
3.2
|
-
|
9.0
|
2.4
|
5.0
|
-
|
11.0
|
2.4
|
5.8
|
-
|
max
|
2.4
|
9.0
|
-
|
-
|
2.4
|
11.0
|
-
|
-
|
2.4
|
max
|
-
|
Purple: Hot bath
Blue: is room temp
Green: warm bath
Red: ice bath
Conclusion- Our results somewhat failed to reject our hypothesis. The hottest condition, the hot water bath, did end up having the most gas produced the quickest and the ice bath had the slowest rate. The tube in the hot water bath actually popped the seal off due to the gas pressure being so high inside the tube from such a high rate of respiration. The tube in the ice bath stayed completely the same after 2 or so minutes, showing us that the cold had either drastically slowed or even stopped respiration in the tube. The two that puzzled us are the room temp and bead bath. An idea which may be the answer to this situation is that the room temp test tube was completely covered by a styrofoam holder, which may have acted as an insulator and helped retain heat. The tube in the bead bath, however, only had about an inch of the tube covered, possibly letting heat escape where it was not covered, or just not heating the entire tube, only the area which was submerged. Another potential source for error could have been one of our systems losing a seal, letting gas escape and not giving us an accurate reading of how much gas was produced.
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